P16. WATER DROP

OBJECTIVES:

• Observe drops of diferents waters (water fishbowl and water of river) 

MATERIAL:

• Water fishbowl

• Water river

• Microspcope

• dropper

• slide and coverslide

PROCEDURES: 

1. prepare two samples of different water drops.

2. put the drops slide the cover and copper objects

3. and observe the bacterias, arthropots and protozoans

P15. PRBB

The group I belonged to had to do the behavior experiment, in which we washed the plates with some water in order to gather the C. elegans. Afterwards we pick them up with a dropper and once they were classified we left them a few minutes so the C. elegans settle.
Then we remove the water and put the C. elegans in a plate with salty water in one side and distilled in the other. If the C. elegans move to the salty water side that means that they smell so they have DAF18, if not they have it silenced.

This is table where we have all the information of the other experiments:

	Microscopy	Image	Genetic	Behavior
ADAF18 mutated	8 male100 hermaphrodite	Intestine NO Pharynx NO	Short	DAF18 mutated
BGFP+RPF	0 male100 hermaphrodite	Intestine YES Pharynx YES	Long	DAF18 mutated
CWild	6 male100 hermaphrodite	Intestine NO Pharynx NO	Long	NO RESULTS
DDAF18 silenced	0 male100 hermaphrodite	Intestine YES Pharynx YES	Long	DAF18 silenced