L5. CARBOHYDRATES

OBJECTIVES:


1. Identify different sugars
2. Understantand the relation between structures and some propeties. 

MATERIAL:

• test tubes
• glucose
• maltose
• sacarose
• lactosa
• starch
• beaker
• bunsen burner
• pipette
• spatula
• lugol
• water destilled
• test-tube rake
• wire gauze

PROCEDURES:

LUGOL	FEHLING
5mL H2O IN A TEST TUBE	2mL H2O
ADD SACCHARIDE	2mL FEHLING A
OBSERVE	2mL FEHLING B
2 DROPS OF LUGOL	TEST TUBE IN A BOLING WATER BATH

RESULTS:

	GLUCOSE	MALTOSE	SUCROSE	LACTOSE	STARCH
SWEET	YES	YES	YES	YES	NO
SOLUBILITY	YES	YES	YES	YES	NO
LUGOL	NEGATIVE	NEGATIVE	NEGATIVE	NEGATIVE	POSITIVE
FEHLING	POSITIVE	POSITIVE	POSITIVE	POSITIVE	NEGATIVE
MONO/DI/POLI	MONO	DI	DI	DI	POLI