P12. ADN

OBJECTIVES:

• Extract and watch banana's DNA

MATERIAL:

• test tubes

• filter paper

• funnel

• 200mL graduated cylinder

• erlenmeyer flask

• 250ml beaker

• spatula

• pipette

• microscope

• soap

• sodium chloride

• H2O

• ethanol 

• acetic orcein 
• pineapple juice

• banana

PROCEDURES: 

1. Weigh 100g of banana, put it on a dish and crush it 

2. In a beaker add 3g of sodium chloride, 100mL of H2O and 10mL of soap. 

3. Add the banana

4. Strain the contentswith a colander in another beaker. With the spatula mix the dissolution.

          

5. Strain it using filter paper. Put funnel in a 200mL graduated cylinder and the paper on a the funnel 

6. With a pipette, put 5mL of the mixture in graduated cylinder and add 1mL of pineapple juice.

7. With another pipette add 6mL of cold ethanol.

8. With a glass spatula remove some filaments and put them in a microscope slide and add a drop of acetic orcein 

QUESTIONS:

1. How do you get rid of the membranes surrounding DNA? by denaturation

2. Why use pineapple juice? use it because it is a good denaturant

3. How to get scattered precipitation of DNA? because the membrane that protects the DNA is destroyed

PHOTOS: